Effect Of Different Egg Yolk Levels On The Quality Of Frozen Buffalo Bull Semen
By: Afzal, M
| Rashid Ahmed Chaudry.
Contributor(s): Imtiaz Hussain Khan | Muhammed | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 1992Subject(s): Department of TheriogenologyDDC classification: 0322,T Dissertation note: The comparative study of various egg yolk levels in the extenders used for deep freezing of buffalo semen was conducted at Semen Production Unit, Qadirabad (Sahiwal). The routinely used Lactose-Fructose-Glycerol semen extender was added with 20, 15, 10, 5 and 0% egg yolk concentration in five experimental extenders. 50 ejaculates collected from 10 buffalo bulls were frozen in Liquid Nitrogen. Each semen sample from all bulls and egg yolk levels was evaluated for post-thaw sperm motility, livability and absolute index of livability.
The average values obtained from egg yolk levels and bulls for all test parameters were found statistically high (P < 0.01) at 10 percent egg yolk level as compared to all others. The order of merit for post-thaw motility, livability and the absolute index of livability at 10, 20, 15, 5 and 0% egg yolk level was 45.9%, 43.5%, 42.9%, 33.7% and 8.7% for motility, 9.8, 9.34, 9.3, 7.68 and 2.75 (Hours) for livability and 231.6, 207.95, 204.85, 123.25 and 13.85 for livability index, respectively.
The comparative mean values obtained from each bull were observed non-significant for post-thaw sperm motility but were found significantly higher (P < 0.05) among bulls regarding livability and the absolute index of livability.
On the basis of the results obtained in the present study it may be concluded that due to synergestic effect between glycerol and the presence of 10% concentration of egg yolk in the extender, being comparatively more economical, exhibiting better visibility and on account of high values obtained from motility, livability and absolute index of livability, an extender containing 10% egg yolk level is recommended for the preservation and deep freezing of buffalo bull semen in Liquid Nitrogen for obtaining optimal semen quality.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 0322,T (Browse shelf) | Available | 0322,T |
The comparative study of various egg yolk levels in the extenders used for deep freezing of buffalo semen was conducted at Semen Production Unit, Qadirabad (Sahiwal). The routinely used Lactose-Fructose-Glycerol semen extender was added with 20, 15, 10, 5 and 0% egg yolk concentration in five experimental extenders. 50 ejaculates collected from 10 buffalo bulls were frozen in Liquid Nitrogen. Each semen sample from all bulls and egg yolk levels was evaluated for post-thaw sperm motility, livability and absolute index of livability.
The average values obtained from egg yolk levels and bulls for all test parameters were found statistically high (P < 0.01) at 10 percent egg yolk level as compared to all others. The order of merit for post-thaw motility, livability and the absolute index of livability at 10, 20, 15, 5 and 0% egg yolk level was 45.9%, 43.5%, 42.9%, 33.7% and 8.7% for motility, 9.8, 9.34, 9.3, 7.68 and 2.75 (Hours) for livability and 231.6, 207.95, 204.85, 123.25 and 13.85 for livability index, respectively.
The comparative mean values obtained from each bull were observed non-significant for post-thaw sperm motility but were found significantly higher (P < 0.05) among bulls regarding livability and the absolute index of livability.
On the basis of the results obtained in the present study it may be concluded that due to synergestic effect between glycerol and the presence of 10% concentration of egg yolk in the extender, being comparatively more economical, exhibiting better visibility and on account of high values obtained from motility, livability and absolute index of livability, an extender containing 10% egg yolk level is recommended for the preservation and deep freezing of buffalo bull semen in Liquid Nitrogen for obtaining optimal semen quality.
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